If you have encountered a problem with BLAST+ (stand-alone BLAST ® applications), you may first want to:

If the above does not help, please write to us at: Ideally, we would like to receive detailed information that will allow us to reproduce and investigate the problem. The items that we need are:

1. Summary: a brief, but specific description of the problem (for example, use "blastn crash with outfmt 7" instead of "blastn crashes")

2. Version of BLAST: This can be obtained by running any BLAST+ application with the -version-full argument: 

$ blastn -version-full
blastn: 2.15.0+
 Package: blast 2.15.0, build Oct 19 2023 15:45:46
 Build-Signature:  GCC_730-ReleaseMT64--x86_64-unknown-linux3.10.0-gnu2.17-coremake22
 GI-64bit:  TRUE
 Build-Date:  Oct 19 2023 16:29:50
 Revision:  674175
 Subversion-Revision:  674175
 Stable-Components-Version:  28
 Development-Version:  20230606
 Built-As:  blastn


3. DetailsProvide us with steps that we can follow to reproduce the problem, including command line arguments, inputs, and outputs.  It would especially help us if you:

  • Set the environment variables DIAG_TRACE=1 and DIAG_POST_LEVEL=Trace prior to running the command that fails (see examples below).
  • Capture the output and send it along with your report.

Examples of setting the DIAG_TRACE/DIAG_POST_LEVEL variables:

For bash or zsh shell
export DIAG_TRACE=1
export DIAG_POST_LEVEL=Trace


For csh/tcsh shell
setenv DIAG_TRACE 1
setenv DIAG_POST_LEVEL Trace


4. System information:

For UNIX-like operating systems, use the output of the following command:

For Linux OSs
uname -a
ulimit -a


For macOS
sysctl -a


For Windows operating systems:

  • Access the Start menu (All Programs) by pressing the window key on your keyboard.
  • Locate the Run... command (Figure 1). 
  • If not already entered, type msinfo32 and press the Enter key.
  • In the resulting window, click File, then Export... 
  • Save the file that contains the operating system information and provide the file to us.  If you need more help, see the Microsoft site that addresses obtaining system information


A small web pop-up titled as "Run" with an entry box containing the "msinfo32" text.

Figure 1: The Run command.

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