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  • What are GenBank accession numbers and what information is embedded in their format?

  • How do I set up Nucleotide BLAST (blastn) to analyze coding regions (CDS) on sequences that I am submitting to GenBank?

  • How do I use Nucleotide BLAST (blastn) and CDS feature display to determine if I have nucleotide insertions or deletions in my sequence that may cause reading frame shifts?

  • How do I use Nucleotide BLAST (blastn) and CDS feature display to determine if a protein-coding sequence has poor quality or incorrect nucleotide substitutions?

  • How do I associate my virus sequences in GenBank with my data in the Sequence Read Archive (SRA)?

  • I have sequenced an entire genome (transcriptome). How do I submit it to GenBank?

  • Will I be able to keep my submitted sequence/molecular data confidential at NCBI until I publish my paper?

  • Why am I getting an error message stating that the GenBank submission system (BankIt) is unavailable?

  • How do I use Nucleotide BLAST (blastn) with CDS feature display to determine the coding strand (plus or minus) on my sequences?

  • How do I use Nucleotide BLAST (blastn) and the CDS feature display to determine the coding locations for eukaryotic genes (those with intron/exon structure)?

  • How do I change the release date of my GenBank and Sequence Read Archive (SRA) records?

  • How do I interpret Nucleotide BLAST (blastn) pairwise alignments with the CDS feature display?

  • Why do I get error messages when I try to annotate a coding region (CDS) in BankIt?

  • How do I use Nucleotide BLAST (blastn) to determine the coding locations on a sequence from a prokaryotic genome?

  • How are GenBank accession numbers assigned to my submission?

  • How do I find the release date and/or any update date of a GenBank (Nucleotide) sequence record?

  • What kind of sequences can I submit to GenBank using BankIt?

  • Where and how do I submit individual human variation data associated with phenotypes at NCBI?

  • How do I use Nucleotide BLAST (blastn) with CDS feature display to determine the reading frame of a 5’ partial CDS?

  • How do I use Nucleotide BLAST (blastn) with CDS feature display to determine if there are sequence quality problems at the ends of my sequences?

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