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BLAST

  • Why does my BLAST search get a “CPU usage limit was exceeded” error?

  • How do I search a eukaryotic genome with BLAST?

  • How do I reduce search load to avoid being penalized for heavy usage when teaching BLAST in a classroom?

    Measures to help avoid usage penalty when using BLAST in a class setting

  • How do I report BLAST+ problems?

  • What is a BLAST request ID (RID) and why do I need it?

    Request ID for BLAST search

  • How can I bookmark a BLAST search page?

    How to use the "bookmark page" button to share/store blast search settings

  • Why don't I see the Entrez Query option when I select a protein database?

  • How do I download and share BLAST search-strategy files?

    Steps to download search strategies BLAST searches, as well as steps to upload a search strategy file to regenerate the search settings

  • Does "blastdb_aliastool" support the Accession-Based BLAST databases?

  • How many sequences can I enter in a single BLAST search?

  • Why doesn't BLAST find my sequence even though it is in GenBank?

  • How does BLAST penalize heavy usage from an IP address?

    BLAST usage limit and monitoring

  • Does "update_blastdb.pl" support the Accession-Based BLAST databases?

  • Why does my BLAST search get a “Process size limit exceeded” error?

    Ways to handle process size limit errors

  • How can I limit standalone BLAST+ searches by organism?

  • How can I see more than 100 matching sequences in my BLAST search results?

  • How do I use BLAST for 16S ribosomal RNA sequences to identify or verify prokaryotic organisms?

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